Sunday, July 13
| Time | Event |
|---|---|
| 12 – 5 p.m. | Check in at University Tower Apartments, 4500 Cass Ave, Detroit, MI 48201 |
| 5 – 5:10 p.m. | Opening Remarks, Arun Anantharam, Ph.D. General Lectures Building, 5045 Anthony Wayne Dr. |
| Plenary 1 5:10 – 5:55 p.m. | James Rothman, Ph.D., Yale University, USA New Insights into Basic Mechanisms of Synaptic Transmission |
| Plenary 2 6 – 6:45 p.m. | Jenny Hinshaw, Ph.D., NIH, USA Cryo-EM of Dynamin Assemblies Bound to Endocytic-Relevant Phosphoinositides in Different Nucleotide States |
| 7 – 9 p.m. | Reception (dinner provided), St. Andrew’s Hall, 918 Ludington Mall |
Monday, July 14
| Time | Event |
|---|---|
| 7:30 – 8:30 a.m. | Breakfast |
| Plenary 3 8:30 – 9:15 a.m. | Robert Edwards, M.D., Ph.D., University of California, San Francisco, USA The Biogenesis of Dense Core Vesicles |
| 9:15 – 9:35 a.m. | David Njus, Ph.D., Wayne State University, USA Neuromelanin Precursors and the Unique Vulnerability of Dopaminergic Neurons in Parkinson’s Disease |
| 9:35 – 9:50 a.m. | Break |
Session 1: Mechanisms of Exocytosis and Endocytosis I
Chairs: Ira Milosevic, Ph.D., and Prabhodh Abbineni, Ph.D.
| Time | Event |
|---|---|
| 9:50 – 10:10 a.m. | Jeremy Dittman, M.D., Ph.D., Weill-Cornell Medical College, USA Assembling a Fusion Machine: Insights into the Synaptic Hub Protein Munc13 |
| 10:10 – 10:30 a.m. | Volker Kiessling, Ph.D., University of Virginia, USA Lipid-Protein and Protein-Protein Interactions Guiding SNARE-Mediated Membrane Fusion |
| 10:30 – 10:50 a.m. | Jihong Bai, Ph.D., Fred Hutchinson Cancer Institute, USA Synaptic Vesicle Endocytosis: Roles of Amphipathic Motifs |
| 10:50 – 11:10 a.m. | Michael Cousin, Ph.D., University of Edinburgh, UK Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis |
| 11:10 – 11:25 a.m. | Break |
Session 2: Chromogranins I
Chairs: Teresa Pasqua, Ph.D. and Sushil Mahata, Ph.D.
| Time | Event |
|---|---|
| 11:25 – 11:45 a.m. | Teresa Pasqua, Ph.D., University of Calabria, Italy Cardioprotective Effects of Novel Catestatin Peptides |
| 11:45 a.m. – 12:05 p.m. | Ennio Avolio, Ph.D., University of Calabria, Italy Vasostatin-1 Restores Autistic Disorders in an Idiopathic Autism Model (BTBR T+ Itpr3tf/J mice) by Decreasing Hippocampal Neuroinflammation |
| 12:05 – 12:25 p.m. | Sushil Mahata, Ph.D., UC San Diego, USA Catestatin Modulates Adrenergic Signaling to Suppress Tauopathy and Improve Cognition in PS19 Mice |
| 12:30 – 1:30 p.m. | Lunch |
Session 3: Intracellular Trafficking
Chairs: Rajesh Ramachandran, Ph.D. and Kevin Bohannon, Ph.D.
| Time | Event |
|---|---|
| 1:30 – 1:50 p.m. | Tina Lee, Ph.D., Carnegie Mellon University Regulation of ER membrane fusion by human Atlastin GTPases |
| 1:50 – 2:10 p.m. | Kevin Bohannon, Ph.D., University of Michigan Resiliency, remodeling, and repair of the lysosomal membrane is mediated by ESCRTs |
| 2:10 – 2:30 p.m. | Michael Ailion, Ph.D, University of Washington, USA A dense-core vesicle biogenesis pathway that bypasses the Golgi |
| 2:30 – 2:50 p.m. | Rajesh Ramachandran, Ph.D., Case Western Reserve University, USA Coincidence detection in mitochondrial membrane fission |
| 2:50 – 3:05 p.m. | Break |
Session 4: Synaptic Transmission I
Chair: Meyer Jackson, Ph.D.
| Time | Event |
|---|---|
| 3:05 – 3:25 p.m. | Vitaly Klyachko, Ph.D., Washington University in St. Louis, USA Spatiotemporal regulation of synaptic release sites |
| 3:25 – 3:45 p.m. | Valentina Carabelli, Ph.D., University of Torino, Italy Exogenous alpha-synuclein slows-down action potential firing and enhance dopamine release in Substantia Nigra dopamine neurons |
| 3:45 – 4:05 p.m. | Skyler Jackman, Ph.D., Vollum Institute, USA Synaptotagmin 3 and 7 are Plasma Membrane Calcium Sensors that Regulate Vesicle Fusion |
| 4:05 – 4:25 p.m. | Meyer Jackson, Ph.D., University of Wisconsin, USA Synaptic fusion pores and quantal release |
| 4:25 – 4:40 p.m. | Break |
Session 5: Aquaporin-4 Vesicles in Health and Disease
Chair: Robert Zorec, Ph.D.
| Time | Event |
|---|---|
| 4:40 – 5 p.m. | Antonio Frigeri, Ph.D. TBD |
| 5 – 5:20 p.m. | Jernej Jorgacevski, Ph.D., University of Ljubljana, Slovenia Vesicle Mobility and Plectin Coordinate Aquaporin 4 Localization and Water Homeostasis in Astrocytes and Glioblastoma |
| 5:20 – 5:40 p.m. | Robert Zorec, Ph.D., University of Ljubljana, Slovenia Adrenergic regulation of astrocyte vesicle traffic in health and disease |
| 5:40 – 6:40 p.m. | Poster Session I |
| 6:40 p.m. | Dinner (on your own) and free time |
Tuesday, July 15
| Time | Event |
|---|---|
| 7:30 – 8:30 a.m. | Breakfast |
| Plenary 4 8:30 – 9:15 a.m. | Lukas Tamm, Ph.D., University of Virginia, USA Control of insulin secretion by synaptotagmin isoforms and membrane lipids under healthy and diabetic conditions |
Session 6: Insulin granule trafficking and secretion
Chair: Jeff Knight, Ph.D.
| Time | Event |
|---|---|
| 9:20 – 9:40 a.m. | Cedric Asensio, Ph.D., University of Denver, USA Sorting of synaptotagmins to the insulin secretory granule |
| 9:40 – 10:00 a.m. | Xuelin Lou, Ph.D., Medical College of Wisconsin, USA Unresolved mysteries of membrane trafficking in β cells: dynamin function and diabetes |
| 10:00 – 10:20 a.m. | Sathish Ramakrishnan, Ph.D., Yale University, USA SNARE Complex Dynamics in Insulin Vesicle Fusion and Its Role in Aging and Metabolic Disorders |
| 10:20 – 10:40 a.m. | Jeff Knight, Ph.D., University of Colorado, Denver, USA Carbonyl reactivity of C2 domain lysine clusters: Impacts on protein function and insulin secretion |
| 10:40 – 10:55 a.m. | Break |
Session 7: Signaling and Secretion
Chair: Paul Kammermeier, Ph.D.
| Time | Event |
|---|---|
| 10:55 – 11:15 a.m. | Heidi Hamm, Ph.D., Vanderbilt University, USA Investigating the molecular mechanism of Gβγ-mediated inhibition of exocytosis through binding the SNARE complex |
| 11:15 – 11:35 a.m. | Matt Brody, Ph.D., University of Michigan, USA Molecular Regulation of Cardiomyocyte Exocytosis and Atrial Natriuretic Peptide Secretion by the Rab3 Cycle |
| 11:35 – 11:55 p.m. | Lee Eiden, Ph.D., NIH, USA PACAP as a neuropeptide co-transmitter in autonomic and central nervous systems at synapses specialized for stress |
| 12 – 3 p.m. | Lunch and free time |
Session 8: Chromogranins II
Chairs: Sushil Mahata, Ph.D. and Nitish Mahapatra, Ph.D.
| Time | Event |
|---|---|
| 3 – 3:20 p.m. | Geert van den Bogaart, Ph.D., University of Groningen, Netherlands Inflammation promotes proteolytic processing of the prohormone chromogranin A |
| 3:20 – 3:40 p.m. | Sushma Dagar, Ph.D., National Brain Research Center, Manesar, India Functional status of Regulated Exocytosis in Obesity-Induced Metabolic Stress |
| 3:40 – 4 | Nitish Mahapatra, Ph.D., IIT Madras, India Exploring the role of Chromogranin A in early-onset myocardial infarction |
| 4 – 4:20 p.m. | Sushil Mahata, Ph.D., University of California, San Diego, USA Catestatin Restores Cardiac Metabolic Flexibility and Mitochondrial Function by Reversing Insulin Resistance |
| 4:20 – 4:35 p.m. | Break |
Session 9: Post pre-fusion and Pre post-fusion – the state-of-the-art for structural studies on intermediates of the membrane fusion of viral infections
Chair: Joshua Zimmerberg, M.D., Ph.D.
| Time | Event |
|---|---|
| 4:35 – 4:55 p.m. | TBD |
| 4:55 – 5:15 p.m. | Tijana Ivanovic, Ph.D., NIH, USA TBD |
| 5:15 – 5:35 p.m. | Walther Mothes, Ph.D., Yale University, USA Viral spike – receptor interaction and membrane fusion monitored by cryo-electron tomography |
| 5:35 – 5:55 p.m. | Joshua Zimmerberg, M.D., Ph.D., NIH, USA Adventures with a.m.phipathic Peptides as Fusogens and Fusion Inhibitors |
Wednesday, July 16
| Time | Event |
|---|---|
| 7:30 – 8:30 a.m. | Breakfast |
| Plenary 5 8:30 – 9:15 a.m. | Nathalie Guerineau, Ph.D., University of Montpellier, France Stress-driven remodeling of stimulus-secretion coupling in the adrenal medulla |
Session 10: Ion Channels and Secretion
Chair: Emilio Carbone, Ph.D.
| Time | Event |
|---|---|
| 9:20 – 9:40 a.m. | Geoffrey Pitt, M.D., PhD., Weill-Cornell Medical College, USA Cav1.2 channels and hypoglycemia in Timothy syndrome |
| 9:40 – 10:00 a.m. | Christopher Lingle, Ph.D., Washington University in St. Louis, USA Nav channel Long-Term Inactivation defined by iFGF-A isoforms limits AP firing rates in CCs and other excitable cells |
| 10:00 – 10:20 a.m. | David Giovannucci, Ph.D., University of Toledo, USA PACAP remodels chromaffin cell excitability and secretory activity |
| 10:20 – 10:40 a.m. | Emilio Carbone, Ph.D., University of Turin, Italy TBD |
| 10:40 – 10:55 a.m. | Break |
Session 11: O’Connor Early Career Awards Symposium
Chairs: Lee Eiden, Ph.D., and Uri Ashery, Ph.D.
| Time | Event |
|---|---|
| 10:55 – 11:00 a.m. | Introduction |
| 11:00 – 11:15 a.m. | Stefanie Geisler, Ph.D., University of Innsbruck, Austria Genetic deletion of Stac2 affects electrical activity of chromaffin cells and mouse behavior |
| 11:15 – 11:30 a.m. | Elke Muntjewerff, Ph.D., Uppsala University, Sweden Neuropeptide Catestatin modulates nerve-immune related inflammation |
| 11:30 – 11:45 a.m. | Anna De Bartolo, Ph.D., University of Calabria, Italy The Chromogranin A-derived fragment (CgA 344–358), Cateslytin, alleviates lipopolysaccharide (LPS)-induced inflammation and injury in cardiomyocytes by inhibiting the MD-2/TLR4 interaction |
| 11:45 a.m. – 12 noon | Quanfeng Zhang, Ph.D., Peking University, China Neurotransmitter Release and Regulation through Fusion Pore at Single Vesicle Level |
| 12 – 1 p.m. | Lunch |
Session 12: Regulators of Chromaffin Cell Secretion I
Chair: Corey Smith, Ph.D.
| Time | Event |
|---|---|
| 1 – 1:20 p.m. | Sam Chen, Ph.D., University of Toledo, USA Molecular mechanisms underlying PACAP-mediated chromaffin cell secretion |
| 1:20 – 1:40 p.m. | Kevin Currie, Ph.D., Cooper Medical College of Rowan University, USA Control of catecholamine secretion from adrenal chromaffin cells by the serotonin transporter |
| 1:40 – 2 p.m. | Luis Miguel Guttierrez, Ph.D., Miguel Hernandez University, Spain Membrane and organelle fusion caused by the Sphingosine derivative FTY-720 in Chromaffin cells |
| 2 – 2:20 p.m. | Elizabeth Seward, Ph.D., University of Sheffield, UK GPCR regulation of Exocytosis in Chromaffin Cells – In Context |
| 2:40 – 2:55 p.m. | Break |
Session 13: Nanoscale Dynamics from Priming to Fusion
Chair: Manfred Lindau, Ph.D.
| Time | Event |
|---|---|
| 2:55 – 3:15 p.m. | Sebastian Barg, Ph.D., Uppsala University, Sweden Dynamic recruitment of Munc13 primes docked secretory granules for exocytosis |
| 3:15 – 3:35 p.m. | Debasis Das, Ph.D., Tata Institute, Mumbai, India Role of fusion pores in quantal size variation – implications in cellular plasticity? |
| 3:35 – 3:55 p.m. | Adekunle Bademosi, Ph.D., Queensland Brain Institute, Australia Dissection of Unc13A synaptic function at the nanoscale resolution |
| 3:55 – 4:15 p.m. | Maria Bykhovskaia, Ph.D., Wayne State University, USA Molecular dynamics of the pre-fusion protein-lipid complex |
| 4:15 – 4:30 p.m. | Break |
Session 14: Synaptic Transmission II
Chair: Andrew Frank, Ph.D.
| Time | Event |
|---|---|
| 4:30 – 4:50 p.m. | Kiel Ormerod, Ph.D., University of Toledo, USA A drosophila model for neuropeptide sorting, capture and release |
| 4:50 – 5:10 p.m. | Kathrin Engisch, Ph.D., Wright State University, USA Homeostatic Synaptic Plasticity of quantal size in Mouse Cortical Cultures Requires Neuronal Rab3A |
| 5:10 – 5:30 p.m. | Andrew Frank, Ph.D., University of Iowa, USA Mitochondrial dysfunction at synapses triggers a ROS-based homeostatic response |
| 5:30 – 6:30 p.m. | Posters II |
| 6:30 – end | Dinner (on your own) and free time |
Thursday, July 17
| Time | Event |
|---|---|
| 7:30 – 8:30 a.m. | Breakfast |
| Plenary 6 8:30 – 9:15 a.m. | Ling-Gang Wu, Ph.D., NIH, USA Exo- and endocytosis: membrane transformations, molecular mechanics and functions |
Session 15: Regulators of Chromaffin Cell Secretion II
Chair: Elizabeth Seward, Ph.D.
| Time | Event |
|---|---|
| 9:20 – 9:40 a.m. | Fernando Marengo, Ph.D., University of Buenos Aires, Argentina Dynamin-2 mutations linked to neonatal-onset centronuclear myopathy impair exocytosis and endocytosis in adrenal chromaffin cells |
| 9:40 – 10:00 a.m. | Yongsoo Park, Ph.D., Hamad Bin Khalifa University (HBKU), Qatar PIP2 Is An Electrostatic Catalyst for Vesicle Fusion by Lowering the Hydration Energy: Arresting Vesicle Fusion by Masking PIP2 |
| 10:00 – 10:20 a.m. | Thomas Ferrand, University of Rouen, France Chromogranin A / Phosphatidic acid interaction governs the regulated exocytosis in chromaffin cells |
| 10:20 – 10:40 a.m. | Normand Leblanc, Ph.D., University of Nevada, Reno, USA Novel role of L-type voltage-gated Ca2+ channels in Na+ accumulation triggered by high intensity nanosecond electric pulses in adrenal chromaffin cells |
| 10:40 – 10:55 a.m. | Break |
Session 16: SNARE-opathies
Chair: Jacqueline Burre, Ph.D.
| Time | Event |
|---|---|
| 10:55 – 11:15 a.m. | Ege Kavalali, Ph.D., Vanderbilt University, USA Insights into synapse nanostructure from analysis of SNARE disease variants |
| 11:15 – 11:35 a.m. | Shyam Krishnakumar, Ph.D., Yale University, USA Molecular Characterization of VAMP2-associated Epileptic Phenotypes |
| 11:35 – 11:55 p.m. | Manu Sharma, Ph.D., Weill-Cornell Medical College, USA How Tau Exits the Neurons |
| 11:55 – 12:15 p.m. | Jacqueline Burre, Ph.D., Weill-Cornell Medical College, USA Syntaxin dysfunction in Munc18-1/STXBP1 Encephalopathies |
| 12:15 – 1:15 p.m. | Lunch |
Session 17: Secretory Vesicles
Chair: Ricardo Borges, M.D., Ph.D., and David Njus, Ph.D.
| Time | Event |
|---|---|
| 1:15 – 1:35 p.m. | Nicolas Vitale, Ph.D., University of Strasbourg, France Subunits of the V0 segment of V-ATPase interact with the SNARE complex and regulate the synthesis of fusogenic lipids at the exocytotic sites |
| 1:35 – 1:55 p.m. | Ira Milosevic, Ph.D., University of Oxford, UK New modes of the v-ATPase activity regulation in neurons and neuroendocrine cells |
| 1:55 – 2:15 p.m. | Hui Gu, Ph.D., University of Gothenburg, Sweden Amperometry and Electron Microscopy show Stress Granules Induce Homotypic Fusion of Catecholamine Vesicles |
| 2:15 – 2:35 p.m. | Ricardo Borges, M.D., Ph.D., University of La Laguna, Spain Delta granules in platelets vs chromaffin granules |
| 2:35 – 2:50 p.m. | Break |
Session 18: Mechanisms of Exocytosis and Endocytosis II
Chair: Ruth Heidelberger, M.D., Ph.D.
| Time | Event |
|---|---|
| 2:50 – 3:10 p.m. | Huan Bao, Ph.D., University of Virginia, USA Differential regulation of SNARE-mediated membrane fusion by annexins and TDP-43 |
| 3:10 – 3:30 p.m. | Ruth Heidelberger, M.D., Ph.D., University of Texas, Houston, USA Roles of syntaxin 3 in membrane fusion and neurotransmitter release |
| 3:30 – 3:50 p.m. | Dixon Woodbury, Ph.D., Brigham Young University, USA Structural changes in isoforms of SNAP-25 due to oxidation and adduct formation |
| 3:50 – 4:05 p.m. | Break |
Session 19: Emerging Nano-biotechnologies
Chairs: Dekel Rosenfeld, Ph.D., and Valentina Carabelli, Ph.D.
| Time | Event |
|---|---|
| 4:05 – 4:25 p.m. | Ann-Sofie Cans, Ph.D., Chalmers University of Technology, Sweden Recording of Fusion Pore Regulated Exocytotic Release of Acetylcholine and Glutamate |
| 4:25 – 4:45 p.m. | Ute Becherer, Ph.D., University of Saarland, Germany Highly adaptable deep-learning platform for automated detection and analysis of vesicle exocytosis |
| 4:45 – 5:05 p.m. | Manfred Lindau, Ph.D., University of Miami, USA Electrochemical Detector arrays in the study of exocytosis in chromaffin cells |
| 5:05 – 5:25 p.m. | Dekel Rosenfeld, Ph.D., Tel Aviv University, Israel Emerging nanotechnologies to facilitate remote modulation of chromaffin cells |
| 5:25 – 5:35 p.m. | Closing remarks |
| 6 p.m. | Dinner (provided) – Elephant and Company Tap Room 456 Charlotte Street, Detroit, MI 48201 |