Congratulations to Lauren Kotsull and Mary Kay H. Pflum on their newly published research, “Thermal shift engagement assay for Class IIA histone deacetylase inhibitor screening.” In this research article, the authors developed an efficient method known as thermal shift assays to directly assess inhibitor binding for HDAC4, 5, and 7. Additionally, a higher throughput thermal shift format was developed using dot blot analysis. This thermal shift analysis offers a useful complement to enzyme activity assays for characterization of HDAC inhibitors. You can read more about the research here:
Results in Chemistry, 2025 Volume 18, 102682, ISSN 2211-7156.
https://doi.org/10.1016/j.rechem.2025.102682.